Ferrous sulphate tablet is an oral salt of iron. It is the cheapest form of medicinal iron supplement and it is used in the treatment of iron deficiency anaemia. Iron deficiency is one of the world’s most common nutritional deficiency diseases, affecting infants, children and especially pregnant women as well as lactating mothers. Various brands of ferrous sulphate tablets were assayed to confirm the amount of iron content as claimed by the manufacturers, so as to ensure maximum therapeutic effects during clinical use. Seven different brands of ferrous sulphate tablets by different manufacturers which were obtained from various pharmacy outlets in Delta State and Anambra of Nigeria were assayed using the titrimetric method of analysis as described by the British Pharmacopoeia (BP) 2012. They were also tested for uniformity of weight by the method stated in the BP. All the samples were found to contain iron content in an amount within the percentage content of 95.0% - 105.0%. Also, no variation was found in the weight of the samples. Therefore, these brands when administered can bring about maximum therapeutic benefits during clinical use.




                Assay methods in pharmaceutical chemistry are essential to control the quality of pharmaceutical product, as well as the quantity of the active pharmaceutical ingredients in a formulated product. To do this, a whole arsenal of chemical, physicochemical, biological, biopharmaceutical and automated analytical techniques is employed for determining the identity, purity, content, stability, safety and efficacy of drugs and their formulations. [1]

                Different forms of iron are usually prescribed by physicians for patients and they rely on the amount of iron claimed on the label. Determination of the precise amount of iron (II) contained in ferrous sulphate tablet is therefore important. Ferrous sulphate tablet has been assayed using various analytical methods.


                Iron has for long been considered important for the body. Lauha bhasma (calcined iron) has been used in ancient Indian medicine. According to Greek thought, Mars is the god of strength and iron is dedicated to mars: thus iron was used for weakness which is common in anaemia. [2]

In 1713, iron was shown to be present in blood. In the early 19th century, Blaud developed his famous Blaud’s pill consisting of ferrous sulphate and potassium carbonate for anaemia. [2]


                There are various forms of iron present in oral formulations and they are: ferrous succinate (35% iron), iron choline citrate, iron calcium complex (5% iron), ferric ammonium citrate (scale iron), ferrous amminoate (10% iron), ferric glycerophosphate, iron hydroxyl polymatose, ferrous fumarate (33% iron), ferrous gluconate (12% iron), ferrous sulphate (hydrated salt 20% iron, dried salt 30% iron) and colloidal ferric hydroxide (50% iron).[2]

                Dissociable ferrous salts are inexpensive, have high iron content and are better absorbed than ferric salts, especially at higher doses. Ferrous sulphate tablet which is an oral salt of iron is the cheapest and is sometimes preferred in the treatment of iron deficiency on this account. [2]

                Iron, forms the nucleus of the iron-porphyrin haem ring which together with globin chain forms haemoglobin. Haem is a tetrapyrrole and iron is at its centre. Haemoglobin reversibly binds oxygen and provides the critical mechanism for oxygen delivery from the lungs to other tissues. [3]

                 Iron is also an essential component of myoglobin; haem enzymes such as the cytochromes, catalase and peroxidase; and the metalloflavoprotein enzymes including xanthine oxidase and the mitochondrial enzyme alpha-glycerophosphate oxidase. [4]


               Iron deficiency anaemia (microcytic hypochromic anaemia) as the name implies is the absence of adequate iron, as a result of which small erythrocytes with insufficient haemoglobin are formed. [3] Without enough iron, the body uses up all its stored iron in the liver, bone marrow and other organs. Once the stored iron is depleted, the body then makes very few red blood cells resulting to Iron deficiency anaemia. [5]


                Iron deficiency anaemia is usually caused by blood loss, poor diet and inadequate iron absorption. The most common cause of iron deficiency in adult is blood loss and the gastrointestinal tract is the common site of blood loss. [3]


                 In iron deficiency anaemia, the erythrocyte mean cell volume (MCV) is less than 2273ml and the mean cell haemoglobin concentration (MCHC) is low (less than 30%). [3]

                Iron deficiency anaemia leads to pallor, fatigue, dizziness, exertional dyspnea, and other generalized symptoms of tissue hypoxia. There is also an unusual craving for non-nutritive substances such as ice, dirt, paint or starch. Some patients can also develop a strong urge to move the legs (the restless legs syndrome). [5]

                Iron deficiency can affect metabolism in muscle independently of the effect of anaemia on oxygen delivery. This may reflect a reduction in the activity of iron-independent mitochondrial enzymes. [4]


                Iron deficiency is the most common cause of chronic anaemia. Iron deficiency has been associated with behavioural and learning problems in children, abnormalities in catecholamine metabolism and possibly impaired heat production.

                In infants and children, there is impaired motor development and coordination, impaired language development, decreased physical activity, psychological and behavioural effects. [6]

                 In adults, there is decreased physical work and earning capacity and resistance to fatigue. [4]

                In pregnant women, there is increased maternal, fetal morbidity and mortality, as well as increased low birth weight.  Awareness of the ubiquitous role of iron has stimulated considerable interest in the early and accurate detection of iron deficiency and its prevention. [4]


                Iron deficiency is commonly seen in populations with increased iron requirements. These include infants, especially premature infants; children during rapid growth periods; pregnant and lactating women. [3] It is worth highlighting that the pregnant population is among the highest risk segment for iron deficiency. [7] Iron deficiency can also be seen in patients with chronic kidney diseases, who lose erythrocytes at a relatively high rate, and also form them at a high rate as a result of treatment with the erythrocyte growth factor, erythropoietin.


                Several laboratory tests can confirm the presence of iron deficiency anaemia. The most commonly used are those that measure serum ferritin, transferrin saturation and erythrocyte protoporphyrin.

                Serum ferritin can be measured by radioimmunoassay (RIA) or enzyme linked immunoassay (ELISA). At all ages, a serum ferritin value of less than 10-12µg/L, indicates a depletion of iron stores.

                Transferrin saturation is calculated by measuring both serum iron and total iron binding capacity using spectrophotometric technique. The iron concentration is divided by the iron binding capacity and multiplying by 100 to express the result as a percentage. In adults, values below 16% is indicative of iron deficiency, while for infants and children values below 12% and 14%, respectively, is indicative of iron deficiency.

                Erythrocyte protoporphyrin accumulates in red blood cells when it has insufficient iron to combine with to form haem. It can be measured by a fluorescence assay performed directly on a thin film of blood. If the value is higher than 80µg/dl of red blood cells for children below the age of four and 70µg/dl for children above the age of four, then there is iron deficiency anaemia.


                Approaches to its prevention include; supplementation with medicinal iron, education and associated measure to increase dietary iron intake and the control of infection such as that caused by parasitic worms. Measures to increase dietary iron intake include taking food such as liver, egg yolk etc. which are very rich in iron. In the control of infection, there should be provision of safe water, improvement in environmental sanitation and personal hygiene. All these will help improve iron status.


Food like liver, kidney, spinach, and egg yolk are rich in iron, but sometimes it is necessary to supplement the diet with “iron tablets”. [8] Iron deficiency anaemia is therefore, treated with oral (ferrous sulphate tablet) or parenteral iron preparations (iron dextran or iron sorbitol). Oral iron corrects the anaemia just as rapidly and completely as parenteral iron in most cases if, iron absorption from the gastrointestinal tract is normal. But for patients with chronic kidney disease, parenteral iron administration is preferred. [3]

                 In an iron-deficient individual, about 50-100 mg of iron can be incorporated into haemoglobin daily and about 25% of oral iron given as ferrous salt can be absorbed. Therefore, 200-400mg of elemental iron should be given daily to correct iron deficiency most rapidly. Treatment with oral iron should be continued for 3-6 months after correction of the cause of the iron loss. This corrects the anaemia and replenishes iron stores. [3]

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How To Write Chapter Three Of Your Research Project (Research Methodology)

  • Methodology In Research Paper

    Chapter three of the research project or the research methodology is another significant part of the research project writing. In developing the chapter three of the research project, you state the purpose of research, research method you wish to adopt, the instruments to be used, where you will collect your data, types of data collection, and how you collected it.

    This chapter explains the different methods to be used in the research project. Here you mention the procedures and strategies you will employ in the study such as research design, study design in research, research area (area of the study), the population of the study, etc. You also tell the reader your research design methods, why you chose a particular method, method of analysis, how you planned to analyze your data.

    Your methodology should be written in a simple language such that other researchers can follow the method and arrive at the same conclusion or findings.

    You can choose a survey design when you want to survey a particular location or behavior by administering instruments such as structured questionnaires, interviews, or experimental; if you intend manipulating some variables.

    The purpose of chapter three (research methodology) is to give an experienced investigator enough information to replicate the study. Some supervisors do not understand this and require students to write what is in effect, a textbook.

    A research design is used to structure the research and to show how all of the major parts of the research project, including the sample, measures, and methods of assignment, work together to address the central research questions in the study. The chapter three should begin with a paragraph reiterating the purpose of research. It is very important that before choosing design methods try and ask yourself the following questions: Will I generate enough information that will help me to solve the research problem by adopting this method?

    Method vs Methodology

    I think the most appropriate in methods versus methodology is to think in terms of their inter-connectedness and relationship between both. You should not beging thinking so much about research methods without thinking of developing a research methodology.

    Metodologia or methodology is the consideration of your research objectives and the most effective method and approach to meet those objectives. That is to say that methodology in research paper is the first step in planning a research project work.

    Design Methodology: Methodological Approach

    Example of methodology in research paper, you are attempting to identify the influence of personality on a road accident, you may wish to look at different personality types, you may also look at accident records from the FRSC, you may also wish to look at the personality of drivers that are accident victims, once you adopt this method, you are already doing a survey, and that becomes your metodologia or methodology.

    Your methodology should aim to provide you with the information to allow you to come to some conclusions about the personalities that are susceptible to a road accident or those personality types that are likely to have a road accident.

    The following subjects may or may not be in the order required by a particular institution of higher education, but all of the subjects constitute a defensible in metodologia or methodology chapter.

    Click here to complete this article - How To Write Chapter Three Of Your Research Project (Research Methodology)


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