EFFECTS OF VERNONIA AMYGDALINA ETHYL ACETATE FRACTION ON INTESTINAL GLUCOSE ABSORPTION IN ALLOXAN INDUCED DIABETIC ADULT WISTAR RATS


Department Of Chemical Engineering


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EFFECTS OF VERNONIA AMYGDALINA ETHYL ACETATE FRACTION ON INTESTINAL GLUCOSE ABSORPTION IN ALLOXAN INDUCED DIABETIC ADULT WISTAR RATS

ABSTRACT  

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and hyperlipidemia caused by insulin deficiency or insulin resistance. This study evaluated the effects of ethyl acetate fraction of Vernonia Amygdalina extract on glucose absorption in alloxan-induced diabetes in adult Wistar rats. Twenty (20) rats weighing between 150g -180g were used for the study and were grouped into four groups (n=5). Group 1 (control) was only fed with growers' mash and water daily. Group 2 was diabetic control. Group 3 rats were non-diabetic and treated with 1ml of Vernonia Amygdalina extract. Group 4 rats were diabetic and treated with 1ml of Vernonia Amygdalina extract. 40mg/kg intraperitoneal injection of Alloxan solution was used to induce diabetes mellitus in this animal model. After 8days of Vernonia Amygdalina administration, glucose absorption in the small intestine was determined by everted sac method where a part of the small intestine was inserted into a Kreb’s bicarbonate solution in an aerated flask and incubated for 30 minutes and the glucose absorption by the tissue is accessed by spectrophotometer. Data from this study were analyzed using Statistical Package for Social Sciences (SPSS version 21) software and expressed as Mean ± Standard Error of the mean (SEM) using ANOVA with the significant level at PVenonia Amygdalina extract. This may be attributed to the inhibitory action of Vernonia Amygdalina on glucose transporters (SLGT-1 and GLUT-2) while subsequently increasing sodium ion (Na+) transport. Conclusively, Vernonia Amygdalina has hypoglycemic potential which was evidenced in the reduced blood glucose level observed in this study.

 

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PAGES: 58
FORMAT: MS WORD
Chapters: 1-5
PRICE: ₦ 5,000.00




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