The isolation and identification of bacteria associated with  150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients.  The  sample collected are wound pus and wound exudates which were collected from patients with diabetic ulcer, surgery wounds, sickle cell ulcers, gun shot wound, using sterile stent striates to  aspirate the wound exudates and steete swab sticks to swab the wound prof. Population was sampled

 According to wound type and noting their differences  sex distributing palternage range (0-2_ years, (3-5_ years (608) years (9-11_ years, (12-14) years, (15-17) years, (18-20) years, and 21 years and above, in sex distribution. (male and female), in predisposing factors and in any other factor affecting it and also their anatibiotic sensitivity pattern.  The samples collected were plated out to on Mac conkey agar, Blood agar, Perory citrate agar (DCA) and further examination was done such as motility test gram staining and some brolhemoceltest like citrate, on and catalyset test foar confirmation.  The results obtained from 250 patients smaples, 199 were positive pathogens and 51 were negative out of which 97 (48.890) were staphylococcus aureus, Staphylococcus aureus was arecorded as the organism that was the highest prevelence while galmorella species has the lowest prevalence 3(1.5%) In this study.  The sensivity patttern of the isolated organisms results show that pseudomonas aeruginosa is resistant to more drugs than the staphylococcus aureus and escherichris coli.  Msot organisms were sensitive ato ciprofloxacillin, Amplicillin, Oxfloxacillin,  Erythroylin, Cotrimoxazole.


Title page





Table of contents

List of tables

List of figures


1. Introduction

1.1 complication

1. Aim and objectives

2. Statement of problems

3. The significance of study

4. Hypothesis\

5. Scope of study

6. Limitation


2. Literature review

2.1 general incidence to wound sepsis

1. Actiological agends of wound sepsis

2. Diagnosis and pathogenesis of wound sepsis

3. Epideomology of wound sepsis

4. Age and sex incidence

5. Predisposing factor of wound sepsis

6. Chemo therapy of wound sepsis


3. Materials and methods

3.1 materials and culture media used

1. Collection and processing sample

2. Method

3. Biochemical characterization isolates

4. Gram staining

5. Catalase test

6. Coagulase test

7. Motilidy test

8. Oxidse test

9. Indole test

10. Methyl red test

11. Phenylative deamination test

12. Citrase utilization test

13. Antibigram




5.0 Discussion, suggestions, conclusions and recommendation.

1. Discussion

2. Suggestion

3. Conclusions

4. Recommendation


1. Subjects smapled accoridng to inpatients, out patients, age and sex distribution

2. Subjects sampled according to types of wounds, age and sex distribution 

3. Paterns of organisms isolated in wounds sepsis in Enugu

4. Age and sex distribution of isolates in wound sepsis

5. Bacterial pathogens causing wound sepsis in patients with sickle cell disease in Enugu

6. Pattern of bacterial pathogens causing wound sepsis in patients with surgical wounds.

7. Bacterial isolated in patients with Gunshot wound sepsis

8. Bacterial isolated from patients with diabetes ulcers

9. Antibiogram of bacterial isolates from wound sepsis in Enugu

10. Biochemical reactions and identifications and identification test on bacterial isolates from wound sepsis in Enugu


1. showing organisms isolated from wound sepsis in Enugu (piechart)

2. Bacterial pathogenesis causing wound sepsis in patients with sickle cell disease

3. Bacterial pathogens causing wound sepsis in the patients with surgical wounds (Barcharts)

4. Bacterial isolates from patients with gunshot wound (bar chart)

5. Bacterial isolates from patients with diabetic ulcer (Bar chart)



Wound is any interruption, by violence or by surgery, in the continuity of the external surfaces of the body or of the surface of any internal or pan (madonald, 1990). According to him, legally, the whole thickness of the skin must be broken, and creating an internal injury wound.  Is also a breach of a coetaneous, mucous or serious surface (Charles 1979). Wound can also be defied as injury to the skin or underlying tissues or organs by a blow or cut, missile or stab which includes injury to the skin caused by chemicals,  cold, friction, heat, pressure and rays, and manifestation in the skin of internal conditions such as pressure sure and ulcers (Roper 1989).

Wound sepsis is the infection of wound by phylogenic organisms (Roper, 1989).  Wounds can be divided into Many types which includes (a) Incised wounds:  which are produced by sharp scuttling instruments (There is aponeuetic fascia of the scalp and most operations wounds are of a this category, Lacerased wounds which may be produced in category, Lacerased wounds which may be produced in road accidents, by factory machinery.  This type of wound is frequently contaminated and it supplies an excellent culture medium for microorganisms.  There is more pain than in an incised wound but bleeding may not be severe due to constriction of blood vessels.  Punctured wound which may be inflicted by sharp instrument, nails, edge’s teeth, knives and bullets.  These wounds are specially susceptible to injection from anaerobic organisms as those causing tetanus and gas gangrene which start thriving when the aerotic organisms such as staphylococcus and streptococcus have used up the available oxygen in the deep tissues.

Poisoned wounds are those which occurs as a result of insect strings, snake bites and dog bites and the inject bites produce swelling.  Irritation and dis-comfort caused and devitalized wounds are those that result for  industrial and severe road accidents and the area and depth of devitalized tissue depends upon the area and weight of the coushing force and the duration and velocity of impact.

Burns and scalids which results from the destruction of tissue by dry heat fraction, electricity radiation or corrosive fluid while scald results from the destruction of tissue by mist heat.  Bruise and confusion is a superficient injury without damage to the skin and the swelling, pain and dis comfort are dye to the extra-vacation of blood into tissues.  Colour changes occur as a result of the moglobin oxidatron.

Haematoma is the collection of blood in the tissues which causes a swelling which when pressed on surrounding structures, the haemotoma become readily injected by micro-organism

Sprain is another wound type which involves the tearing of the capsule and ligaments round a joint with subsequent exudation of fluids.

Wounds  cab further be classified into clean wounds with the amount of contamination being up 30% or more in dirty wounds.  The class I (clean) wounds which are non traumatic with no break in surgical technique without any septic folus or viscera being opened.  Classs II (clean contaminated) wounds are non traumatic, with only minor breaker own in technique being allowed orentry into a vucous without significant spillage.  The class III (contaminated) are traumatic wounds froma relatively clean source, or with a major break in technique or significant spillage from an open viscous, or when acute non- purulent infection is encountered.  Class IV (dirty) wounds are frammatic wounds from a dirty source following delayed treatment or when acute bacterial contamination and releases of pus occur.

Micro organism of clinical importance can be isolated from wounds and they include Achinomyces species.  Bacteriodes species, clostridium perfringes, Eschericha coli, other gram negative enteric bacilli, my cobacterium species, pseudomonas aerug, nwa proteus vulgaaris, staphlococcus aureus. Stapholococcus epidermis stretococcus faecalis, clostridium fetani (Fischachi, 1987)

Wound sepis depends on the opportunities for infection in different parts of the body which in turn depends on the normal bacteria flora of the part, the size of the wound, duration of the operation increase in the length of stay in the hospital and can even result in patients death


Complication arising from wound sepsis are suppurations which results when there is an  imbalance between the infective agent on one hand and the defensive reaction on the other.  The other complications are cellulite which is characterized by a spreading infection, necrosis and sloughing; septicemia, pyaemia and kaolin formation chilling worth et al 1979).  Others that can also result from wound sepsis are gas gangrene, clostridia mystics, clostridical crepitate celluslitis which is a type of mixed infection usually found as a complication of wounds characterized by necrosis of the aveolar and fascia tissues with progressive gangrenous changes in the skin secondary in thrombosis or nutrient vessels repetition of the wound result from the formation of gas by the bacteria synergistic gangrene which is a mixed infection caused by the synergistic action of the acrobic hemolytic staphylococcus aurous and micro Europhilic haemolytic streptococcus also occur as a senous complication peritonitis, perforation and absences formation are other complications.  Wound sepsis can be complicated by mixed infection in which yeast and often fungal infection are involved (sabistan 1981) .  other complications are diabetic micro antipathy which involves the small vessels and capillaries an further complicate diabetes Nellutus.

Distinct and definitive thickening of the usement member inflammatory changes occur lading to the impairment of the resistant of the skin to secondary infections and delay the rate of healing following injures.  Arkrosclerosis is another major complication of diabetes mellitus.  Corponary Vessels are affected producing myscardid infection are the  vessels of the lower extremities producing gangrene of the toes and feets.  The precipitating causes of gangrene of the lower extremities resulting in ulceration, infliction and subbasement gangrene..

In the case of sickle cell disease leg ulcers, there is general reduction in the oxygen carrying capacity of blood resulting.  In tissue dypoxia  and parenthesis of the extremities occurs but although certain compensatory adjustment of the circulation occur the patients can become immemorially compromised in cases of secondary infections due to blocking of small vessels by sickled cells and infare of bones and joints which enhances the continued preponderance of any infecting organism (Anderson 1985).

This work is therefore an attempt to investigate bacteria associated with infection of wounds noting their differences .  in distribution peltern, age range 10-2) years, (3-5) years, (6-8) years (9-11) year (12-14) years, (15-17) years, (18-20) years and 21 years and above, sex distribution (male and female) predisposing factor and any other factor affecting in Nigeria where antibiotic sensitivity pattern especially in Nigeria where drugs are takes indiscriminately without prescription


1. The aim of this work is to isolate and identified bacteria associated with wound sepsis

2. And the objectives are to assess whether any correlation exists between age, sex and different types of wound.

2.c To assess the antibiogram of different bacteria associated with wound sepsis so as to offer advice on therapy.


Wound sepsis has really posed a serious threat to both surgical and un-surgical wounds.  There has been prevalence in wound sepsis following iyunes and fome disease state, and may have failed to heal after administration of common antibiotics resulting to foul smelling and petrifying wounds owing to their discriminate use of drugs especially in togena.

This  problems really propelled met know whether it is because efln discriminate use of dmgs that contributed to sensitive organisms to become resisted string to the drugs or could it be because of mosconical injection which comes bystaying very long in the hospital. And why staphylococcus has been the prechominant organisms threatening wounds.


The standard clean sanitary condition is employed to avoid mosconial infection 


HO Staphocollus aureus is predominantly associated with wound sepsis

H1 Staplococcus aureus is not  predominantly associated with wound sepsis

HII: There is correlation between age, sex and different types of wound sepsis.


       The scope of the study is to isolate and identify bacteria associated with 

wound seosis, noting their distribution pattern, age and sex relationship, 

predisposing factor and any other factor affecting it and also their antibiotic 

sensitivity especially in Nigeria where drugs are taken indiscriminately 

without prescription.


This project work has been limited on only surgical, sickle cell, ulcer, 

Diabetic  ulcer  and  gun  short  wound  sepsis.  Collection  of  samples  and 

materials used is limited to National orthopedic hospital Enugu  Metropolis.  

This project is also limited  to  open  wounds  and  to  patients t hat  lactnot 

stayed for a long time, in the  clinic  to  eliminate  nosconial  infection  and 

sense had not used a lot of antibiotic which would have killed the 

opportunistic bacteria.

The significance of study.



RESEARCHWAP.COM is an online repository for free project topics and research materials, articles and custom writing of research works. We’re an online resource centre that provides a vast database for students to access numerous research project topics and materials. guides and assist Postgraduate, Undergraduate and Final Year Students with well researched and quality project topics, topic ideas, research guides and project materials. We’re reliable and trustworthy, and we really understand what is called “time factor”, that is why we’ve simplified the process so that students can get their research projects ready on time. Our platform provides more educational services, such as hiring a writer, research analysis, and software for computer science research and we also seriously adhere to a timely delivery.


Please feel free to carefully review some written and captured responses from our satisfied clients.

  • "Exceptionally outstanding. Highly recommend for all who wish to have effective and excellent project defence. Easily Accessable, Affordable, Effective and effective."

    Debby Henry George, Massachusetts Institute of Technology (MIT), Cambridge, USA.
  • "I saw this website on facebook page and I did not even bother since I was in a hurry to complete my project. But I am totally amazed that when I visited the website and saw the topic I was looking for and I decided to give a try and now I have received it within an hour after ordering the material. Am grateful guys!"

    Hilary Yusuf, United States International University Africa, Nairobi, Kenya.
  • " is a website I recommend to all student and researchers within and outside the country. The web owners are doing great job and I appreciate them for that. Once again, thank you very much "" and God bless you and your business! ."

    Debby Henry George, Massachusetts Institute of Technology (MIT), Cambridge, USA.
  • "I love what you guys are doing, your material guided me well through my research. Thank you for helping me achieve academic success."

    Sampson, University of Nigeria, Nsukka.
  • " is God-sent! I got good grades in my seminar and project with the help of your service, thank you soooooo much."

    Cynthia, Akwa Ibom State University .
  • "Great User Experience, Nice flows and Superb functionalities.The app is indeed a great tech innovation for greasing the wheels of final year, research and other pedagogical related project works. A trial would definitely convince you."

    Lamilare Valentine, Kwame Nkrumah University, Kumasi, Ghana.
  • "Sorry, it was in my spam folder all along, I should have looked it up properly first. Please keep up the good work, your team is quite commited. Am grateful...I will certainly refer my friends too."

    Elizabeth, Obafemi Awolowo University
  • "Am happy the defense went well, thanks to your articles. I may not be able to express how grateful I am for all your assistance, but on my honour, I owe you guys a good number of referrals. Thank you once again."

    Ali Olanrewaju, Lagos State University.
  • "My Dear Researchwap, initially I never believed one can actually do honest business transactions with Nigerians online until i stumbled into your website. You have broken a new legacy of record as far as am concerned. Keep up the good work!"

    Willie Ekereobong, University of Port Harcourt.
  • "WOW, SO IT'S TRUE??!! I can't believe I got this quality work for just 3k...I thought it was scam ooo. I wouldn't mind if it goes for over 5k, its worth it. Thank you!"

    Theressa, Igbinedion University.
  • "I did not see my project topic on your website so I decided to call your customer care number, the attention I got was epic! I got help from the beginning to the end of my project in just 3 days, they even taught me how to defend my project and I got a 'B' at the end. Thank you so much, infact, I owe my graduating well today to you guys...."

    Joseph, Abia state Polytechnic.
  • "My friend told me about ResearchWap website, I doubted her until I saw her receive her full project in less than 15 miniutes, I tried mine too and got it same, right now, am telling everyone in my school about, no one has to suffer any more writing their project. Thank you for making life easy for me and my fellow students... Keep up the good work"

    Christiana, Landmark University .
  • "I wish I knew you guys when I wrote my first degree project, it took so much time and effort then. Now, with just a click of a button, I got my complete project in less than 15 minutes. You guys are too amazing!."

    Musa, Federal University of Technology Minna
  • "I was scared at first when I saw your website but I decided to risk my last 3k and surprisingly I got my complete project in my email box instantly. This is so nice!!!."

    Ali Obafemi, Ibrahim Badamasi Babangida University, Niger State.
  • To contribute to our success story, send us a feedback or please kindly call 2348037664978.
    Then your comment and contact will be published here also with your consent.

    Thank you for choosing