ISOLATION AND IDENTIFICATION OF BACTERIAL FROM NAIRA NOTE


ISOLATION AND IDENTIFICATION OF BACTERIAL FROM NAIRA NOTE  

ABSTRACT

Survey of the currency bills in University of Uyo, for fungal contaminations revealed presence of fungal organisms such as species of Aspergilliusniger, Aspergillus fumigatus, penicillium spp, mucor spp, candida albicans,and Rhizopus spp. This study was carried out using a total of 101 samples of banknotes denomination (N5, N10, N20, N50, N100, N200, N500, and N1000) obtained from different points of collections within University of Uyo. The currencies were processed for fungal isolation using potato dextrose agar medium and streaking technique was employed for the isolation and purification of the colonies. Microscopic and Macroscopic examination revealed that N500 denomination had the highest incidence of organism, and samples from College of Science and Technology buttery were also the most contaminated. Paper money handling should include but not limited to carrying currency notes in wallets, envelops and holding with clean hands. The promotion of a cashless economy is also advocated; Nigerian paper money has been found in this study to be contaminated with Bacteria and could be vehicles for the transmission of fungal diseases of man. 

TABLE OF CONTENTS

Title Page---------

Approval Page--------

Declaration--------

Dedication---------

Acknowledgement-------

Abstract---------

Table of Contents-------

CHAPTER ONE 

INTRODUCTION 

1.2 AIMS AND OBJECTIVES 

1.3 JUSTIFICATION  

CHAPTER TWO 

LITERATURE REVIEW

2.2 HISTORY OF THE NIGERIAN CURRENCY

2.3TYPES OF MONEY 

2.4DENOMINATION 

BANKNOTES

2.5BACTERIA

2.5.1BACTERIA CLASSIFICATION 

CHAPTER THREE

MATERIALS AND METHODS

3.1     EQUIPMENTS 

3.1.1    REAGENTS 

3.1.2   MEDIA/ AGAR 

3.2 METHODS 

3.2.1 Sample collection 

3.2.2 Examination of currencies for contamination by Bacteria

3.2.3 Isolation and Identification of Bacteria

CHAPTER FOUR

RESULTS

4.1.2 GRAPHICAL REPRESENTATION 

CHAPTER FIVE

SUMMARY OF FINDINGS AND CONCLUSION

5.1 DISCUSSION 

5.2 CONCLUSION

REFERENCES

CHAPTER ONE 

INTRODUCTION 

In most day-to-day cash transactions, money, in form of notes and coins, pass through the hands of many people; as a form of payment for goods and services, settlement of debts and for deferred payment in economic activities (Beg and fisher, 1997). Various denomination of the naira notes have been minted by the Central bank of Nigeria (CBN). They are released to the public, through the Commercial banks currently; there are eight denominations of the naira in note form: N5, N10, N20, N50, N100, N200, N500 and N1000 notes. The N5, N10, N20, N50, 

N100 and N200 naira notes are the most common and are more involved daily cash transactions. They are common especially among the populace while the N500 and N1000 notes are commonly used among the wealthy and in corporate transactions (Okon et al., 2003). Individuals handling the notes shed some of their body flora on the notes; leading to the spread of the microorganisms among the handlers. This has been implicated in serious health hazard such as impairment of lungs function (Osim, 1996). The contamination of the notes can be traced to dust, soil, water, micro flora of the body of handlers (hand, skin, etc.), the saliva often used when counting the notes and wounds. Some money handling habits such as: keeping naira notes in brassiere, socks and pockets, under the carpet or rugs and squeezing in the hand frequently introduce microbes to the notes. Citrobacter sppMycobacterium lapiae, Salmonella spp, Shigella spp, Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginosahave been isolated from naira notes (Haque, 2003). 

The contamination of the naira notes could also be from several sources, it could be from the atmosphere, during storage, usage, handling or production (Awodiet al., 2000). Daily transactions have made the naira to pass through many hands and pathogens become imposed on them. Ogo et al., (2004) reported that the source of contamination could be as a result of poor or negative money handling practices like spraying during ceremonies where such notes may be trampled upon when they fall on the ground.Most of them are normal flora of the human skin; however, some e.g. S. aureus and P. aeroginosacan be opportunistic pathogens. This suggests that the notes could serve as formites for some infectious agents. In this study dirty naira notes of different denominations were collected and analysed for their bacteriological quality as indicated by the kinds of bacteria they harbour. 

Parasites are organisms that live in a close relationship with other organisms (hosts) and are capable of causing harm to their host. Parasites that have been observed to be contaminants of the naira notes are mainly of faecal origin (Awodiet al., 2000). When hands used in cleaning up the anus after passing out faeces are not properly washed and are used to touching the naira note in any way, the tendency is contamination with the trophozoite of the developed parasite, eggs, cysts or even the oocyst. Other attitudes such as the wetting of hands or fingers with saliva or use of contaminated water to lubricate the hand in counting money could lead to possible transfer of parasite and bacteria from such medium to the notes (Ameh and Balogun, 1997).  

Bacteria are ubiquitous and their ability to contaminate objects such as the naira notes is very prevalent when compared to other organisms. Ordinarily, the exposure of naira notes to the atmosphere could even bring about contamination depending on the environment in question (Ameh and Balogun, 1997). Since the isolation of bacterial and fungal organisms from naira notes in Zaria (Ameh and Balogun, 1997) and the effect of parasitic and bacterial organisms on man it has become necessary for thorough investigations to be carried out to determine safety of the naira note.  

Microbial Contamination of paper money is not confined to developing nations. Several studies from the United States reported contamination of coins and paper bills and the identification revealed the presence of pathogenic microbes like Staphylococcus aureus, Escherichia coli, Klebsiella enterobacter. Another survey isolated total of 93 different types of bacteria belonging to the genera Staphylococcus, Streptococcus, Enterobacter, Acinetobacter, Pseudomonas, Bacillus, Diptheroids, Klebseillaand Escherichia. 

1.2 AIMS AND OBJECTIVES 

The aim of this study is to investigate the likelihood of microbial contamination of Nigerian currency in circulationin University of Uyo. Knowledge of the microbial diversity of currency notes in circulation can provide the basis for improved health consciousness in people during currency handling and effective control of transmission of infections. 

This research was carried out: 

⦁ To isolate and characterize Bacteria from paper note samples collected in University of Uyo

⦁ To check for the most prevalent BacteriainUniversity of Uyo

⦁ To show the area with the most contaminated paper notes 

⦁ To identify ways of control, enlightenment and eradication of these disease-causing organisms. 

1.3 JUSTIFICATION  

Bacteria are a diverse group of microorganisms which are of various importance in food and health sciences and they also a major important pathogenic organism, their spores can survive dryness and this study is to isolate and characterize Bacteria found on the Nigerian currency in University of Uyoand how these organisms can affect the health of populace of this institution. 

CHAPTER TWO 

LITERATURE REVIEW

2.2 HISTORY OF THE NIGERIAN CURRENCY

Until the formal introduction of British coins in Nigeria towards the end of the nineteenth century and banknotes much later in the second decade of the twentieth century, the pre-colonial economy had witnessed the use of what some analysts have described as trade currencies. Others have preferred to call them local currencies (Jones 1977; CBN, 1979). In either case or both, the important thing to be underlined is that these expressions acknowledge the fact that these currencies existed and were used for economic transactions among the people. The currencies in question included copper wires, brass rods, manilas, cowry shells, among others. According to some earliest historical records, different kinds of currencies such as copper wires and brass rods were extensively used as a standard currency in the Oil Rivers Province of Nigeria well into the first half of the twentieth century. Also, in use as a currency was the ironbar, which featured prominently in the Eastern Delta of modern Nigeria, as well as the interior of Eastern Nigeria. By the second half of the nineteenth century the iron bar had served as a standard measure of imports and exports in the region. To this end, all other forms of currencies in use there were related to the bar as a standard unit of measure. For instance, 40 manilas were said to have exchanged for the bar as at 1820(Jones 1977). In one of his writings, James Barbort estimated that in the seventeenth century, the iron bar was a major currency employed in the trade of the Rio Real region by the Portuguese merchants. He also said that by 1699 the iron bar replaced the copper bracelets which had by 1508been described by Pacheco Pereia as highly prized(Jones 1977).Besides the use of the iron bar in parts of the Eastern Delta and the Rio Real regions, it was also said to have been extended to the interior of Eastern Nigeria in the early years of the twentieth century. In an apparent reference to an earlier study done by Hutchinson, (2003) Jones underscores that the iron bar was employed as a subsidiary currency unit in the 1930s, in some areas of Eastern Nigeria. These areas included the Nkummu, the Akajuk and Ukelle areas in the Ogoja Province. Here, the currency took the form of a flattened strip of metal shaped like Y with along tail (Jones 1977). It has further been argued that this currency may have penetrated these communities from the Rio Real (considered to be the common entrance between the New Calabar and the Bonny Rivers) where it dominated trade in 1699. The Portuguese merchants had used it as a medium of exchange for the purchase of African slaves in the Rio Real during the era of the trans-Atlantic slave trade (Jones, 1977). Manilla was another major currency of precolonial Nigeria. It promoted economic relations in the Oil Rivers Province and the Rio Real. However, it has been argued that the currency survived into the colonial period (Kirk- Greene, 

1960; McPhee, 1970). Manillas were made of alloy. They were horseshoe shaped in nature (Grey, 1951; Kirk-Greene, 1960). In a letter to the Foreign Office in London in 1858, Consul T. J. Hutchinson had described the Manilla as a piece of copper of a horse-shoe form, measuring about four inches the circumference of their circle (Jones, 1963). In an essay, G.I. Jones has remarked that the Manilla circulated widely in the Oil Rivers region and the Rio Real. He also remarked that the Portuguese trade in the Rio Real area consisted in the exchange of copper in the form of bracelets (Manillas) for the slaves. He added that as at the time of O. Dapper and the Dutch monopoly, Manillas were specifically described as the local currency and the copper rods were traded for slaves (Birthwistle, 1913). Outside the Rio Real, manilla is known to have gained acceptance and wider circulation in the suburbs of the Oil Rivers Protectorate. The areas included Opobo, Bonny, new Calabar, Uyo, Abak, Ikot Ekpene, Eket, Bende, and other parts of the Igbo-speaking country of Nigeria (Birthwistle, 1913). As in 1906, the “Atorni” or “Wa-ahono” manila prevailed in parts of Bonny, Opobo and Akwete (Talbot, 1967). Other forms of the manilla, going by their local names, were categorised as follows (Talbot, 1967): 

i. The “Ama-Ogono-Igbiki” manilla (town money) used extensively in the new Calabardistrict.

ii. The “Prince” manilla, used especially in new Calabar and Okrika.

iii. The “Perekule” or “Ono-odo” manilla used in Akwete, Ohambele, Asa and Obunio.

iv. The “Awo’-nawo” manilla, used more particularly in new Calabar

v. The Jajamanilla, used at Opobo; its value was the same as that of the Okuu-Kpawmanilla, that is, the manilla of Jumbo of Bonny. 

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ISOLATION AND IDENTIFICATION OF BACTERIAL FROM NAIRA NOTE



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