AMELIORATIVE EFFECT OF SELENIUM YEAST ON SOME PHYSIOLOGICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

AMELIORATIVE EFFECT OF SELENIUM YEAST ON SOME PHYSIOLOGICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

ABSTRACT

Oxidative stress and lipid peroxidation are central factors in the metabolic dysfunctions and pathologies associated with diabetes. The results from studies on the benefits of Selenium a trace element with antioxidant, anti-lipidemic and anti-inflammatory properties, in diabetes mellitus have been controversial without prospective outcome and Se appears to be a double-edged sword in the pathologies of diabetes mellitus. It was suggested that selenium could cause glucose disturbance and increase the risk for diabetes mellitus. The present study intends to determine the ameliorative effects of selenium yeast on blood glucose level, oxidative stress and lipid peroxidation biomarkers, and abnormal lipid profile, serum levels of liver enzymes, electrolytes, triiodothyronine and tetraiodothyronine levels in streptozotocin induced diabetes in Wister rats. Thirty five (35) adult male Wistar rats weighing (180 – 200) grams randomly divided into six treatment and one control groups of five rats each (n = 5). Hyperglycemia was induced in all groups except Group IV by single intraperitoneal injection of 60mg/kg of streptozotocin dissolved in 0.1ml fresh cold citrate buffer pH 4.5 into 16 h-fasted rats. In addition, Groups I and II received 0.1 and 0.2 mg/kg/day for 4weeks of selenium yeast respectively, Group III received 1mg/kg/day for 4weeks of glibenclamide, Groups IV and V served as the normal and diabetic control groups respectively and received only 0.9% of normal saline. Groups VI and VII received 300 and 120 mg/kg/day for 4weeks of aspirin and ibuprofen respectively, all treatments were administered via oral route. Blood samples were collected from the tail vein on weekly basis for the period of 4weeks and used for determination of blood plasma glucose levels, and at the end of the fourth week rats were euthanized and blood samples were drawn from the heart by cardiac puncture and used to estimate oxidative stress biomakers (i.e. superoxide dismutase, catalase and gluthation peroxidase) and lipid peroxidation biomarkers (i.e. malondealdehyde),lipid profile, serum levels of liver enzymes, electrolytes, triiodothyronine and tetraiodothyronine levels. Analysis of variance and Turkey‟s post-hoc test were used to analyze the data obtained. The results showed that there was significant (P < 0.05) decrease in blood glucose level at week one and week three with the dose of 0.2 mg/kg of selenium yeast administered, while with the dose of 0.1mg/kg of selenium yeast, there was no significant difference in blood plasma glucose level when compared with the diabetic control group. It was also revealed that the serum liver enzymes aspertate amino transferase and alanine amino transferase were significantly higher (P < 0.05) in the groups treated with 0.1 and 0.2 mg/kg of selenium yeast. Also, of the oxidative stress biomarkers assessed, there was significant reduction (P < 0.05) in only the malondealdehyde level of the group treated with 0.2 mg/kg of selenium yeast when compared with the diabetic control group. For the lipid profile assessment, the effect of selenium yeast was only seen in the level of triglyceride in the group treated with 0.2 mg/kg of selenium yeast which was significantly lower (P < 0.05) when compared to the diabetic control. Sodium and chloride ion levels of the serum electrolytes were significantly lowered (P < 0.05) in the group treated with 0.2 mg/kg of selenium yeast when compared to diabetic control group. Serum triiodothyronine and tetraiodothyronine levels did not show any significant difference across all the treated groups when compared to the diabetic and normal control groups. Tissue necrosis factor alpha level in the serum showed a decrease in the groups treated with 0.1 and 0.2 mg/kg of selenium yeast but not statistically significant (P > 0.05) when compared with the normal and diabetic control groups. Therefore, selenium yeast possesses hypoglycaemic property that is comparable to the oral-hypoglycaemic drug glibenclamide. In addition, the effect of the 0.2 mg/kg of selenium yeast on the oxidative stress biomarkers assessed did not provide sufficient evidence to conclude that the selenium yeast used in the study elicited an antioxidant effect. The marked decline in serum triglyceride concentration in the 0.2 mg/kg of selenium yeast treated group was indicative of direct effect of the antioxidant capacity of selenium on oxidation of lipids and lipoproteins.

CHAPTER ONE

INTRODUCTION

1.1   Background to the Study

Diabetes is a common metabolic disorder characterized by hyperglycemia due to an absolute or re1lative insulin deficiency (Lawal et al., 2008; WHO, 2010). It affects essential biochemical pathways of the body including carbohydrate, protein, and lipid metabolisms. The World Health Organization (WHO), estimated that there were 171 million people in the world with diabetes in the year 2008 and this is projected to increase by over a 100% to 366 million by 2030 (WHO, 2010). Diabetes is associated with reduced life expectancy, significant high mortality and diminished quality of life. In 2005 an estimated 1.1 million people died from diabetes and diabetes complications (WHO, 2008). Its prevalence is rising globally, including the rural Nigerian populations (Ime et al.,2011).

Epidemiological reports has highlighted on the fact that low- and middle-income countries will bear the brunt of the increase and that Africa will contribute significantly to this rise. In Africa 40% of people with diabetes live in low and middle income countries causing 5% of the deaths globally each year. This is likely to increase by more than 50% in the next 10 years, if urgent action is not taken (WHO, 2007). The challenges and thus, the solutions in the provision of healthcare that would improve outcome for diabetes in low and middle income countries are many and can be found at multiple levels. Patient-related factors are of extreme importance, these ranges from low levels of self-management practices, lack of adherence to lifestyle changes and medication and lack of faith in the conventional management procedures.

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How To Write Chapter Three Of Your Research Project (Research Methodology)

  • Methodology In Research Paper


    Chapter three of the research project or the research methodology is another significant part of the research project writing. In developing the chapter three of the research project, you state the purpose of research, research method you wish to adopt, the instruments to be used, where you will collect your data, types of data collection, and how you collected it.

    This chapter explains the different methods to be used in the research project. Here you mention the procedures and strategies you will employ in the study such as research design, study design in research, research area (area of the study), the population of the study, etc. You also tell the reader your research design methods, why you chose a particular method, method of analysis, how you planned to analyze your data.

    Your methodology should be written in a simple language such that other researchers can follow the method and arrive at the same conclusion or findings.

    You can choose a survey design when you want to survey a particular location or behavior by administering instruments such as structured questionnaires, interviews, or experimental; if you intend manipulating some variables.

    The purpose of chapter three (research methodology) is to give an experienced investigator enough information to replicate the study. Some supervisors do not understand this and require students to write what is in effect, a textbook.

    A research design is used to structure the research and to show how all of the major parts of the research project, including the sample, measures, and methods of assignment, work together to address the central research questions in the study. The chapter three should begin with a paragraph reiterating the purpose of research. It is very important that before choosing design methods try and ask yourself the following questions: Will I generate enough information that will help me to solve the research problem by adopting this method?

    Method vs Methodology

    I think the most appropriate in methods versus methodology is to think in terms of their inter-connectedness and relationship between both. You should not beging thinking so much about research methods without thinking of developing a research methodology.

    Metodologia or methodology is the consideration of your research objectives and the most effective method and approach to meet those objectives. That is to say that methodology in research paper is the first step in planning a research project work.

    Design Methodology: Methodological Approach

    Example of methodology in research paper, you are attempting to identify the influence of personality on a road accident, you may wish to look at different personality types, you may also look at accident records from the FRSC, you may also wish to look at the personality of drivers that are accident victims, once you adopt this method, you are already doing a survey, and that becomes your metodologia or methodology.

    Your methodology should aim to provide you with the information to allow you to come to some conclusions about the personalities that are susceptible to a road accident or those personality types that are likely to have a road accident.

    The following subjects may or may not be in the order required by a particular institution of higher education, but all of the subjects constitute a defensible in metodologia or methodology chapter.

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