HEPATOPRODUCTIVE EFFECTS OF SENA TORA LEAVE EXTRACT ON CARBON TETRACHLORIDE INDUCED TOXICITY
CHAPTER ONE 1.0 INTRODUCTION
Many of the developing countries including African countries like Nigeria practice traditional medicine as its main source of health care. This traditional medicine is normally gotten from plant origin [Rehan Ahmad et al, 2008, Stephen bent, 2008]. Today nearly 88 percent of the global populations switch to plant-derived medicines as their first line of defence for maintaining health and combating diseases [Kintzois et al,2006]. Presently there are about 60 types of medicinal plants that have been recognised in primary health care and are classified according to their pharmacological actions such as peptic ulcers, anti flatulence, laxative, anti diarrhoea and anti hepatic[Viomolos et al,2003]. However in future the discovery of novel therapeutic agents will be only dependent on plant origin [Perulmalsamy et al, 1999]. Senna Tora is an example of traditional medicine with pharmacological actions as anticholesterolemic, antispasmodic, carminative, emollients, ophthalmic and purgative [Polunin and Stanton,1984,Yeung,1985, Manandhar, 2002]. It can be cardiotonic, antiperiodic, anthelmintic and expectorant and can also be used in treating cough, leprosy, ringworm, colic, flatulence, constipation and other disorders[Nature serve,2007]. Senna tora formally regarded as cassia tora is capable of suppressing the production of prostaglandins and other inflammatory agonists such as cytokines, histamine, kinin and free radical. manila,1998 buttressed his observations confirming its use in the treatment of constipation, edema and liver protection in Korea. The liver, the largest internal organ of the human body performs more than five hundred functions, all of which are very vital to life. The liver can regenerate or grow back cells that have been destroyed by short term injury or disease, but if the liver is damaged repeatedly over a long period of time, it may undergo irreversible changes that permanently interferes with its function. Carbon tetrachloride is a toxic substance that interrupts the liver and its functions. This is to say that it damages the liver when ingested.CCL4 is stable in the presence of air and light, it is also inflammable. Despite its toxic effect on the liver, it is useful as grain fumigant , pesticide, etc. Equilibrium of the body fluids and secretions are altered as a result of carbon tetrachloride. For instance lipid metabolism, cholesterol metabolism and biotransformation functions of the liver are terminated or altered in the presence of CCL4. Thus carbon tetrachloride is lethal. The liver produces some enzymes such as aspartate amino transaminases, alkaline phosphatase, lactate dehydrogenase,gamma-glutamyl transferase etc which catalyzes the biochemical activities of the body, and on the attack of CCL4 to the liver , these enzymes will not be produced. Free radicals are highly reactive compounds with an uneven number of electrons in their outermost orbit. This can react with cellular compounds like unsaturated fatty acids and can generate new free radicals which result in an irreversible biochemical injury like membrane damage, apoptosis, and cell necrosis. Antioxidants scavenge free radicals and stop the subsequent reactions hence protecting the macromolecules and cellular environment from toxicity and degeneration [Hong B.O. Shao et al, 2008]. The oxygen consumption inherent in cell growth leads to the generation of a series of reactive oxygen species [ROS]. The ROS are molecules such as superoxide anion radicals and hydroxyl radicals[OH]. ROS may be very damaging attacking the lipids of the cell membrane and DNA mutation which may further propagate the propagation of many diseases[Valontao et al 2002, Gulcin et al 2003]. Reactive oxygen species are continuously produced during normal Physiological events and are removed by antioxidants defense mechanisms [Buyukokuroglu et al,2001]. Many results have shown that some of the cassia species have acquired antimicrobial substances and antioxidant activities.
1.1 AIMS OF THE RESEARCH The aim of this research is to find out the hepatoprotective effects of senna tora leave extract on carbon tetrachloride-induced toxicity.