SCREENING OF SENNA ALATA LEAVES FOR BIOCHEMICAL AND HYPOGLYCEMIC ACTIVITY
The comparative effects of Sennaalata leaves extract and sulfonylurea drug Glibenclamide on biochemical indices were evaluated in Wistar rats. The design consisted of 4 groups of 6 rats each. Group 1 (NC) and 2 (DC) received placebo, groups 3 (SA) received ethanolic extract of Sennaalata leaves the equivalent of 250mg/kg body weight, while group 4 (GB) was gavaged with 5mg/kg bodyweight of Glibenclamide each administered 12 hourly-twice daily. Treatment lasted for 4weeks (28 days), during which fasting blood glucose of the rats was monitored on the 1st, 7th, 13th, 19th, 25th and 28th day respectively, after which the animals were sacrificed and blood collected for biochemical and hematological analysis. Results of biochemical indices indicated significant (p<0.05) hypoglycemic activity in both treated groups compared to the diabetic group. The reduction in fasting blood glucose for SA (80.6%) and GB (84%) over the treatment, the period was significant (P<0.05) compared to DC (45%). Hepatotoxicity indices (ALT, AST) showed significantly (P<0.05) reduction in AST levels of GB (18.14±5.45) compared to DC (165.62±14.95), although SA (271.17±26.31) was markedly increased when compared to DC. ALT activity was significantly (P<0.05) increased in SA and GB compared to DC. Treatments produced a profound decrease in total proteins in treated animals compared with the controls but the decrease was significant (P<0.05) only when compared with DC. Bodyweight result showed significant (P<0.05) weight gain in both SA and GB diabetic treated groups compared to the DC diabetic untreated group. In conclusion, this study clearly indicates that Sennaalata extract may reverse hyperglycemic states and ameliorate the associated complications in diabetes mellitus and compares favourably with standard drug.