Fresh mature plants of Acalypha wilkesiana were collected from the natural habitat in October within Ambrose Alli University, Ekpoma, Edo State, Nigeria. The plant was authenticated in the Department of Botany, Ambrose Alli University, Ekpoma, Edo State, Nigeria. The leaves were picked, washed in distilled water air dried and weighed. The weight of the leaf was 0.1kg. The dried leaves were pounded to powdered form using a motar and pestle. The leaf powder was suspended in methanol for 24hours before filtering with a white filter cloth and boiled for 30minutes. The resulting methanol extract was allowed to attain room temperature and was evaporated to dryness using a water bath as a source of heat. The percentage yield of the extract was 6.41% (w/v). The pasty residue was used to prepare a standardized solution of the leaf extract in calculated amount of distilled water with standardized concentration of 0.46 g/ml. The standardized solution (the stock solution) was stored air tight in plastic bottles and kept frozen.2.1.2 LABORATORY ANIMALS Exactly twenty four male albino rats weighing 148 ± 30g obtained from the Animal Holding Unit of the Institute of Agricultural Research and Training (IART) Apata-Ibadan, Nigeria were used for the study. The rats were fed with Guinea grower mash (product of bendel feeds and flour mills Ltd, Ewu, Edo state, Nigeria). The rats were grouped into four (4) groups, each groups consisting of six (6) rats.2.2   INSTRUMENTS USED The instruments used for this analysis include;

Beam weigh balance Electronic weigh balance                                                           

2.3 METHODS2.3.1 EXPERIMENTAL ANIMALS AND PROCEDURE The Twenty Four Male albino rats were randomly grouped into four  consisting of six rats per group. The rats were kept in cages made of wooden frames and metal iron and were fed ad libitum (access to food and water for 24hours each day). The rats were fed with guinea grower mash and tap water with 12hours light and dark cycle. The cages were cleaned every morning and disinfected every day. The rats were allowed to acclimatize for seven days before extract administration was commenced. Calculated amount of methanolic leaf extract of Acalypha wilkesiana were constituted in distilled water from the stock solution to give doses of 500,500 and 1000 mg/kg body weight of paracetamol, vitamin C and leaf extract respectively and administered to the various groups as illustrated below; GROUP A: Control received 1ml of distilled water GROUP B: Received 500mg/kg body weight of paracetamol GROUP C: Received 500mg/kg paracetamol and vitamin C GROUP D: Received 500mg/kg paracetamol and 1000mg/kg leaf extract. Prior to the administration of methanolic leaf extract of Acalypha wilkesiana at every interval of seven days, the body weights of the animals were recorded. Administration of methanolic leaf extract of Acalypha wilkesiana was performed orally once daily between 8:30am ± 30 minutes, using metal cannula attached to a 2ml syringe prior to the administration of methanolic leaf extract of Acalypha Wilkesiana at every interval of seven days, the body weights of the animals were recorded. 

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How To Write Chapter Three Of Your Research Project (Research Methodology)

  • Methodology In Research Paper

    Chapter three of the research project or the research methodology is another significant part of the research project writing. In developing the chapter three of the research project, you state the purpose of research, research method you wish to adopt, the instruments to be used, where you will collect your data, types of data collection, and how you collected it.

    This chapter explains the different methods to be used in the research project. Here you mention the procedures and strategies you will employ in the study such as research design, study design in research, research area (area of the study), the population of the study, etc. You also tell the reader your research design methods, why you chose a particular method, method of analysis, how you planned to analyze your data.

    Your methodology should be written in a simple language such that other researchers can follow the method and arrive at the same conclusion or findings.

    You can choose a survey design when you want to survey a particular location or behavior by administering instruments such as structured questionnaires, interviews, or experimental; if you intend manipulating some variables.

    The purpose of chapter three (research methodology) is to give an experienced investigator enough information to replicate the study. Some supervisors do not understand this and require students to write what is in effect, a textbook.

    A research design is used to structure the research and to show how all of the major parts of the research project, including the sample, measures, and methods of assignment, work together to address the central research questions in the study. The chapter three should begin with a paragraph reiterating the purpose of research. It is very important that before choosing design methods try and ask yourself the following questions: Will I generate enough information that will help me to solve the research problem by adopting this method?

    Method vs Methodology

    I think the most appropriate in methods versus methodology is to think in terms of their inter-connectedness and relationship between both. You should not beging thinking so much about research methods without thinking of developing a research methodology.

    Metodologia or methodology is the consideration of your research objectives and the most effective method and approach to meet those objectives. That is to say that methodology in research paper is the first step in planning a research project work.

    Design Methodology: Methodological Approach

    Example of methodology in research paper, you are attempting to identify the influence of personality on a road accident, you may wish to look at different personality types, you may also look at accident records from the FRSC, you may also wish to look at the personality of drivers that are accident victims, once you adopt this method, you are already doing a survey, and that becomes your metodologia or methodology.

    Your methodology should aim to provide you with the information to allow you to come to some conclusions about the personalities that are susceptible to a road accident or those personality types that are likely to have a road accident.

    The following subjects may or may not be in the order required by a particular institution of higher education, but all of the subjects constitute a defensible in metodologia or methodology chapter.

    Click here to complete this article - How To Write Chapter Three Of Your Research Project (Research Methodology)


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