AMELIORATING ROLE OF N.P.K. FERTILIZER ON THE TOXIC EFFECTS OF Ni ON (SORGHUM) ROOT ANTIOXIDANT ENZYMES
TABLE OF CONTENTS
Title Page Certification Dedication Acknowledgment Table of Contents Abstract
CHAPTER ONE
Introduction and Literature Review
1.1 Introduction
Literature Review Definition of heavy metals Characteristics of Nickel Nickel in the environment Biological roles of nickel Absorption of nickel by plant Accumulation of Nickel in plants Nickel and photosynthesis Effects of nickel on plant respiration Metabolic effects of nickel Effects of nickel on enzyme activity Mechanism of nickel toxicity Strategies of plant tolerance to nickel toxicity Management of nickel toxicity Land management procedure
1.2.13.2 Phytoremediation 1.2.14 The use of micro-organisms to mitigate nickel toxicity 22
Scientific classification of Sorghum Chemical Composition and Nutritive Value of
Sorghum 25 1.5 Classification of sorghum 28 1.6 Uses of Sorghum 29 1.7 Germination / Growth Stages of Sorghum 32 1.7.1 Growth Stages 32 1.72 Nutrient Uptake 36 1.8 Diseases of Grain Sorghum 37 1.9 Activities that induce Germination 38 1.10 Metabolism of Germinating Seeds 40 1.11 NPK (15-15-15) Fertilizer 41 1.11.1Catalase 44 1.12 History of Catalase 45 1.12.1 Activities of Catalase 46 1.12.2 Molecular mechanism of catalase action 47 1.13 Superoxide Dismutase 48 1.13.1Types of Superoxide Dismutase 49 1.13.2 Physiological Importance of Superoxide Dismutase 51 1.13.3 Use of Superoxide Dismutase in Cosmetic 52 1.14 Peroxidase 52 1.14.1 Isozymes of Glutathione Peroxidase 53 1.15 Oxidative stress and reactive oxygen species 53 1.16 Objective of Study 56
CHAPTER TWO
Materials and Method 55
2.1 Materials 55 2.1.1 Contaminant 55
Fertilizer 55 Quantity of soil used 55 Source of Soil 55 Source of Soybean seed used 56 Instruments/Apparatus used 56 Reagents used for the study 57 Methods 59 Preparation of Soil 59 Contamination of Soil 59 Viability test of Seeds 59 Experimental design 59 Biochemical analysis 62 Estimation of total protein 61 Estimation of malondialdehyde level 64 Estimation of Superoxide Dismutase activity 66 Estimation of Catalase activity 68 Estimation of peroxidase activity 70
2.2.6 Statistical Analysis 72
CHAPTER THREE
Results 73
3.1 Soil Analysis 78
CHAPTER FOUR
Discussion and Conclusion 79
Bibliography 83 Appendix One: Reagents Preparation 97
Appendix Two: Statistics
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