AMELIORATING ROLE OF N.P.K. FERTILIZER ON THE TOXIC EFFECTS OF Ni ON (SORGHUM) ROOT ANTIOXIDANT ENZYMES

AMELIORATING ROLE OF N.P.K. FERTILIZER ON THE TOXIC EFFECTS OF Ni ON (SORGHUM) ROOT ANTIOXIDANT ENZYMES

TABLE OF CONTENTS  

Title Page                                                                        Certification                                                                 Dedication                                                                     Acknowledgment                                                          Table of Contents                                                           Abstract                                                                   

CHAPTER ONE

Introduction and Literature Review                        

1.1   Introduction                                                           

Literature Review                                                     Definition of heavy metals                                             Characteristics of Nickel                                          Nickel in the environment                                          Biological roles of nickel                                        Absorption of nickel by plant                                  Accumulation of Nickel in plants                             Nickel and photosynthesis                                      Effects of nickel on plant respiration                               Metabolic effects of nickel                                                Effects of nickel on enzyme activity                 Mechanism of nickel toxicity                           Strategies of plant tolerance to nickel toxicity  Management of nickel toxicity                         Land management procedure                         

1.2.13.2   Phytoremediation                                             1.2.14      The use of micro-organisms to mitigate nickel toxicity                                                            22

Scientific classification of Sorghum                     Chemical Composition and Nutritive Value of

Sorghum                                                                  25 1.5   Classification of sorghum                                                28 1.6   Uses of Sorghum                                                     29 1.7   Germination / Growth Stages of Sorghum                       32 1.7.1  Growth Stages                                                               32 1.72 Nutrient Uptake                                                       36 1.8   Diseases of Grain Sorghum                                     37 1.9   Activities that induce Germination                          38 1.10 Metabolism of Germinating Seeds                           40 1.11 NPK (15-15-15) Fertilizer                                         41 1.11.1Catalase                                                                 44 1.12 History of Catalase                                                   45 1.12.1  Activities of Catalase                                            46 1.12.2 Molecular mechanism of catalase action                      47 1.13 Superoxide Dismutase                                             48 1.13.1Types of Superoxide Dismutase                             49 1.13.2 Physiological Importance of Superoxide Dismutase      51 1.13.3 Use of Superoxide Dismutase in Cosmetic            52 1.14 Peroxidase                                                               52 1.14.1 Isozymes of Glutathione Peroxidase                      53 1.15 Oxidative stress and reactive oxygen species           53 1.16 Objective of Study                                              56

CHAPTER TWO

Materials and Method                                              55

2.1   Materials                                                                  55 2.1.1        Contaminant                                                           55

Fertilizer                                                                  55 Quantity of soil used                                                        55 Source of Soil                                                          55 Source of Soybean seed used                                   56 Instruments/Apparatus used                                  56 Reagents used for the study                                     57 Methods                                                                   59 Preparation of Soil                                                   59 Contamination of Soil                                              59 Viability test of Seeds                                               59 Experimental design                                                        59 Biochemical analysis                                                       62 Estimation of total protein                                   61 Estimation of malondialdehyde level                   64 Estimation of Superoxide Dismutase activity               66 Estimation of Catalase activity                             68 Estimation of peroxidase activity                         70

2.2.6          Statistical Analysis                                                        72

CHAPTER THREE

Results                                                                    73

3.1   Soil Analysis                                                            78

CHAPTER FOUR

Discussion and Conclusion                                     79

Bibliography                                                                    83 Appendix One: Reagents Preparation                                       97

Appendix Two:  Statistics

 

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How To Write Chapter Three Of Your Research Project (Research Methodology)

  • Methodology In Research Paper


    Chapter three of the research project or the research methodology is another significant part of the research project writing. In developing the chapter three of the research project, you state the purpose of research, research method you wish to adopt, the instruments to be used, where you will collect your data, types of data collection, and how you collected it.

    This chapter explains the different methods to be used in the research project. Here you mention the procedures and strategies you will employ in the study such as research design, study design in research, research area (area of the study), the population of the study, etc. You also tell the reader your research design methods, why you chose a particular method, method of analysis, how you planned to analyze your data.

    Your methodology should be written in a simple language such that other researchers can follow the method and arrive at the same conclusion or findings.

    You can choose a survey design when you want to survey a particular location or behavior by administering instruments such as structured questionnaires, interviews, or experimental; if you intend manipulating some variables.

    The purpose of chapter three (research methodology) is to give an experienced investigator enough information to replicate the study. Some supervisors do not understand this and require students to write what is in effect, a textbook.

    A research design is used to structure the research and to show how all of the major parts of the research project, including the sample, measures, and methods of assignment, work together to address the central research questions in the study. The chapter three should begin with a paragraph reiterating the purpose of research. It is very important that before choosing design methods try and ask yourself the following questions: Will I generate enough information that will help me to solve the research problem by adopting this method?

    Method vs Methodology

    I think the most appropriate in methods versus methodology is to think in terms of their inter-connectedness and relationship between both. You should not beging thinking so much about research methods without thinking of developing a research methodology.

    Metodologia or methodology is the consideration of your research objectives and the most effective method and approach to meet those objectives. That is to say that methodology in research paper is the first step in planning a research project work.

    Design Methodology: Methodological Approach

    Example of methodology in research paper, you are attempting to identify the influence of personality on a road accident, you may wish to look at different personality types, you may also look at accident records from the FRSC, you may also wish to look at the personality of drivers that are accident victims, once you adopt this method, you are already doing a survey, and that becomes your metodologia or methodology.

    Your methodology should aim to provide you with the information to allow you to come to some conclusions about the personalities that are susceptible to a road accident or those personality types that are likely to have a road accident.

    The following subjects may or may not be in the order required by a particular institution of higher education, but all of the subjects constitute a defensible in metodologia or methodology chapter.

    Click here to complete this article - How To Write Chapter Three Of Your Research Project (Research Methodology)

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