PHYTOCHEMICAL AND ANTIMICROBIAL ANALYSIS OF MISTLETOE LEAVES
TABLE OF CONTENT
CONTENT Title page Declaration Certification Dedication Acknowledgment Table of content Abstract
CHAPTER ONE 1.0 INTRODUCTION AIM AND OBJECTIVES
LITERATURE REVIEW OF COMMON
NAME MISTLE TOE 1.2 PLANT TAXONOMY 1.3 PHYTOCHEMICALS 1.3.1 TANINS 1.3.2 STEROIDS 1.3.3 PHENOLS 1.3.4 SAPONINS 1.3.5 FLAVONOIDS 1.3.6 ALKALOIDS 1.4 MEDICAL USES OF MISTLE TOE CHAPTER TWO 2.0 MATERIALS AND METHODS 2.1 REAGENTS 2.2 CHEMICALS USED 2.3 APPARATUS USED 2.4 PHYTOCHEMICAL METHOD 2.5 PREPARATION OF THE SAMPLE 2.6 ETHANOLIC EXTRACT 2.7 N-HEXANE EXTRACT 2.8 AQEOUS EXTRACT 2.9 ETHYL ACETATE EXTRACT 2.10 CHEMICAL TESTS 2.10.1 TEST FOR ALKAKLOID 2.10.2 TEST FOR TANIN 2.10.3 TEST FOR FLAVONOID 2.10.4 TEST FOR PHENOLS 2.10.5 TEST FOR STEROID 2.10.6 EMULSION TEST 2.10.7 TEST FOR SAPONIN 2.11 EXPERIMENTAL 2.11.1 PREPARATION OF SAMPLE 2.11.2 PREPARATION OF REAGENTS 2.11.3 EXTRACTION OF SAMPLE 2.11.4 AQUEOUS EXTRACT OF THE LEAVE 2.11.5 N-HEXANE EXTRACT OF THE LEAVES 2.11.6 ETHYL ACETATE EXTRACT OF THE LEAVE 2.12 CHEMICAL TESTS 2.12.1 ALKALOID 2.12.3 FLAVONOID 2.12.4 STEROID (SALKOWSKI TEST) 2.12.5 SAPONIN 2.12.6 EMIULSION TEST 2.13 EXTRACTION OF ALKALOIDS 2.14 METHOD OF SEPARATION 2.14 METHODS USED FOR THE TEST AREAS FOLLOWSCHAPTER THREE 3.0 RESULTS 3.1 DISCUSSION ON PHYTOCHEMICAL RESULTS TABLE 1: MINIMAL INHIBITION AND MINIMAL BATERICIDAL CONCENTRATION OF THE FOUR EXTRACTS TABLE 2: MEAN DIAMETER OF ZONE OF INHIBITION (IN MM)CHAPTER FOUR DISCUSSION CONCLUSION RECOMMENDATION REFERENCES
ABSTRACT The sample of Mistletoe leaves was collected from an orange tree at Ururo in Umunze Anambra State. The leaves was extracted by using some solvent like ethanolic solvent, aqueous solvent n-hexane solvent and ethyl acetate solvent. The extracts was further analyzed which helps in determining the presence of phytochemical compounds in the Mistletoe plant leaf, such as alkaloids, flavonoid, phenol, tannins, steroid and saponins. These phytochemical compounds are found to be antimicrobial active agents. The efficiency of this plant in the treatment of various diseases necessitated the present work in order to identify the classes of natural products present in the leaf of the plant. These pharmacological values of this plant in herbal medicine and as base for the development of new drug.
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