EXTRACTION, CHARACTERIZATION AND ANTIMICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL
TABLE OF CONTENT
CONTENT Title page Declaration Certification Dedication Acknowledgment Table of content Abstract
CHAPTER ONE 1.1 INTRODUCTION LITERATURE REVIEW 1.2 TYPES OF OIL 1.2.1 Drying oil 1.2.2 Semi-Drying Oil
NON- DRYING OIL
1.3 COMPOSITION OF OIL 1.4 PIGMENT
1.7 BOTANIC DESCRIPTION
1.8 FUNCTIONAL USES
CHAPTER TWO MATERIALS AND METHODS 2.0 SAMPLE COLLECTION 2.1 OIL EXTRACTION 2.2 DETERMINATION OF SPECIFIC GRAVITY 2.3 DETERMINATION OF DENSITY 2.4 DETERMINATION OF FREE FATTY ACID CONTENT
DETERMINATION OF SAPONIFICATION VALUE DETERMINATION OF IODINE VALUE DETERMINATION OF PEROXIDE VALUE ANTI-MICROBIAL ACTIVITY SCREENING REAGENTS AND MEDIA PREPARATION OF FUNGAL TEST ORGANISM PREPARATION OF THE SENSITIVE TEST AGAR PREPARATION OF THE NUTRIENT DEXTROSE
AGAR 2.8.5 THE PUNCHED AGAR DIFFUSION METHOD AS RECOMMENDED BY BRYANT (1972) 2.7.6 BACTERIAL INOCULATION AND INCUBATION 2.8.7 FUNGAL INOCULATION AND INCUBATION
READING INHIBITION ZONES OF THE OIL
CHAPTER THREE TABLE 3.1 ORGANOLEPTIC CHARACTERISTICS OF Crysophylum albidum SEED OIL 3.2 CHARACTERIZATION OF THE SAMPLE TABLE 3.3 RESULT OF ANTI-BACTERIAL ACTIVITY OF C. albidum OIL ON TWO GRAM POSITIVE BACTERIA TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF C. albidum OIL ON TWO GRAM NEGATIVE BACTERIA TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF C. albidum OIL ON TWO TEST FUNGI 3.6 RESULT OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF C. albidum OIL ON SIX TEST ORGANISM
CHAPTER FOUR 4.0 DISCUSSION 4.1 CONCLUSION
REFERENCES APPENDIX I
ABSTRACTCrysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus 0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50 and A. flavons 0.40. The pharmacological screening confirmed the medical value of this plant oil and it established a good support for the sample in herbal medicine and as a base for the development of new drugs and phytomedicine.
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