PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO)
TABLE OF CONTENT CONTENT Title page Declaration Certification Dedication Acknowledgment Table of content Abstract
CHAPTER ONE 1.0 INTRODUCTION OBJECTIVE OF THIS RESEARCH 1.1 LITERATURE REVIEW 1.2 GENERAL USES AND IMPORTANCE OF MANGIFERA INDICA 1.3 MORPHOLOGY OF MANGIFERA INDICA; THE TREE AND ITS FLOWERS CHAPTER TWO 2.0 MATERIALS AND METHODS 2.1 MATERIALS 2.2 EQUIPMENTS AND APPARATUS 2.3 CHEMICAL REAGENTS 2.4 PHYTOCHEMICAL SCREENING (QUANTITATIVE ANALYSIS) 2.5 DETERMINATION OF SAPONIN 2.6 DETERMINATION OF ALKALOID 2.7 DETERMINATION OF TANINS 2.8 DETERMINATION OF MOISTURE CONTENT 2.9 DETERMINATION OF TOTAL ASH 2.10 CARDIAC GLYCOSIDE 2.11 TEST FOR ANTHRACENE GLYCOSIDES 2.12 TERPENOIDS 2.13 pH AT 200C (10%) CHAPTER THREE TABLE 3.1 ORGANOLEPTIC TEST RESULT TABLE 3.2 RESULT OF THE PHYTOCHEMICAL ANALYSIS OF FLOWER AND STALK OF Mangifera Indica TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM POSITIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM NEGATIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF TWO FUNGI IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica TABLE 3.6 RESULT OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF CRUDE EXTRACT OF FLOWER AND STALK OF Mangifera indica CHAPTER FOUR DISCUSSION CONCLUSION RECOMMENDATION REFERENCES APPENDIX
ABSTRACT Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%, Alkaloid 7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid 6.11% + 0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%, pH 3.84% + 0.0529%. Cardiac Glycoside 7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited..